
Polyacrylamide Gel Electrophoresis (PAGE) is a typical technique for isolating proteins by electrophoresis. In this technique a polyacrylamide gel is utilized as a Base medium, however Sodium dodecyl sulfate (SDS) is utilized for denaturation of proteins chain and proteins. The technique is called as Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (PAGE). Electrophoresis is frequently used to isolate proteins based on the size as well as charge on the nucleolus. A control sample containing desired proteins is stacked onto a permeable matrix and electric voltage is passed through. The proteins in the control sample drift through the matrix at various velocities dependent on their changing size and charge. The matrix used in Polyacrylamide Gel Electrophoresis (PAGE) is made out of numerous materials, for example, paper, cellulose acetic acid derivative and distinctive gels, such as, polyacrylamide, agarose and starch. The Polyacrylamide Gel Electrophoresis (PAGE) kit contains gel, dyes, buffers, pigments, electrophoresis hardware and molecular weight markers. The negative Polyacrylamide Gel Electrophoresis (PAGE) uses separation based on size and shape of micro molecules. The negative Polyacrylamide Gel Electrophoresis (PAGE) method is more prominent protruding used for purification of protein from a mixture