Enzymes are proteins that act as catalyst for the biochemical reactions and metabolic activities in the body. Enzymes help in digestion, muscle contraction and elimination of waste in the body. The normal diet might not provide essential enzymes required for the body, as the cooked and processed foods eliminate the enzyme content. The food enzymes are extracted from animals, plants or by fermentation process involving microorganisms. Modern commercial food enzyme production involves breeding of optimized microorganisms and advanced biotechnology techniques for genetically modification of organisms for obtaining enzymes with desired characteristics. Alpha-amylase, glucoamylase, beta-glucanase, lipase, papin, chymosin, microbial proteases, pectinase, lactase, cellulose, glucose oxidase and some others are major food enzymes. Food enzymes find applications in food industry ,such as breaking down glucose, converting starch to other forms, enhancing flavor and reducing ripening time of cheese, tendering meat, curdling the milk, processing animal and plant protein, processing fruit pulps, additive to dairy products, converting cellulose waste to fermenting feedstock and many others. Enzymes are widely used for the production of alcoholic and nonalcoholic beverages.