Cellpreparation 1. 50,000 cells are counted and harvested, then cells are centrifuged 5 min at 500g, 4?. 2. Remove and discard supernatant. Wash cells with 50 uL cold PBS buffer and centrifuge 5 min at 500g, 4?. 3. Remove and discard supernatant. Resuspend the cell pellet with 50 uL cold lysis buffer centrifuge 10 min at 500g, 4?. 4. Discard the supernatant, the Lyse cells will be generate a crude nuclei. Next, they need immediately continue to transposition reaction.