Real-Time Bioprocess Raman Analyzer is a vibrational spectroscopic technique used to analyze the crystal structure and molecular vibrations. Real-time Bioprocess Raman analyzers are used to measure metabolic and nutrient concentration in bioprocess in real-time by using the single probe. Raman spectroscopy is most promising real-time process analytical technologies in biopharmaceutical production. It allows measuring both quantity and quality of chemical compounds in the liquid where conventional methods fail to measure. Real-time bioprocess Raman analyzers are used to measure gas, liquid, solids substance and provide real-time quantification measurement for glutamine, glucose, lactate, glutamate, ammonium, viable cell density, osmolality, total cell density and cell viability. Real-time bioprocess Raman analyzers consist of following components: light source, fibre optical Raman probe, spectrograph, detector, optical fibre cable and others. Real-time bioprocess Raman analyzers allow the usage of the optical fiber to measure the distance and immersion probe helps to analyze the liquid medium. This makes real-time bioprocess Raman analyzer more superior in analyzing bioprocess components and concentration changes than MIR and NIR.