Nucleic acid isolation and purification is a procedure of molecular biology techniques such as cloning, sequencing, polymerase chain reaction and others. These techniques have various applications in the field of genetic engineering, life science research, forensics and molecular diagnostics. In addition, mRNA isolation and purification, RNA isolation and purification, plasmid DNA isolation and purification, total RNA isolation and purification, and PCR clean up. Nucleic acid isolation helps in processing of more sample in less time, minimizes nucleic acid loss ad degradation and increases laboratory efficiency and effectiveness. It also helps in purification of nucleic acid. Column based, reagent based, magnetic bead based and others are some important technology used in nucleic acid isolation and purification. These are used by hospitals, pharmaceuticals, academic research, diagnostic centers, biotechnology companies and food testing and laboratories.