Whole exome sequencing (WES) refers to a technique for sequencing the coding region of the genome (exon). Whole exome sequencing helps to detect rare variants in the exome for identifying genomic cause of various diseases such as cancer, genetic disorders, monogenic disorders and others. Whole exome sequencing is used by research centers, pharmaceutical companies, hospitals, clinics, biotechnology companies and government institutions for sequencing of genome. Some of the application areas for whole exome sequencing are drug discovery and development, agriculture, cancer, personalized medicine, monogenic disorders, diagnostics and others.